DNA Extraction (guanidine isothiocyanate}
1. Prepare 2-3 ml EDTA-blood sample in 15 ml Falcon tube 2. Add eritrosit lysis buffer up to 5x dilution (8-12 ml) cat: tambah EL buffer ad 5 x misal 2 ml tambal 10 mL 3. Invert several times gently to mix the solution 4. put on ice for 20 minute 5. spin at 750 g for 10 minute 6. discard supernatan; clean up the tube wall by invert (dimiringkan) falcon tube in tissue paper 7. add 100 uL of SE buffer 8. replace solution into of SE buffer (campur dengan menyedot beberapa kali) 9. add 100 uL of guanidine isothyocyanate 4 Molar, mix well by pippeting 10. add 700 uL of chloroform 11. add 400 uL of NaCl 6 Molar 12. Shake strongly by hand (a critical step) 2-3 menit sampai timbul gumpalan yg tetap) 13. spin at 10000 rpm for 10 min after sentrifugation, 3 layer appear in the solution ( transparent in upper (DNA0 and lower part , whereas milk like in the middle part/at wall of the tube (protein) 14. replace upper part of solution in a new1,5 mL tube. use pipet and note the v...